Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System
نویسندگان
چکیده
Michael Krypuy1, Tomasz K. Wojdacz2, and Alexander Dobrovic1,3 1Molecular Pathology Research and Development Laboratory, Department of Pathology, Peter MacCallum Cancer Centre, Melbourne, Australia; 2Institute of Human Genetics, University of Aarhus, Aarhus, Denmark; 3Department of Pathology, University of Melbourne, Parkville, Australia *Corresponding authors: [email protected], [email protected], [email protected]
منابع مشابه
qPCR 2005 2 nd International qPCR Symposium Industrial Exhibition TATAA
1: In recent years, capillary-based LightCycler® Systems have become an established standard for rapid and accurate real-time PCR. Based on innovative technology for heating/cooling and highly accurate analysis algorithms, they enable thermal cycling at high speed and with precisely controlled temperatures, characteristics directly related to the consistency and accuracy of scientific results. ...
متن کاملUtility of the MagNA Pure 96 and LightCycler<sup></sup> 480 Systems for Testing of Stool Samples for Toxin-producing <i>Clostridium difficile</i>
Clostridium difficile infection (CDI) is an important cause of hospital diarrhea. Conventional CDI testing by enzyme immunoassays is fast yet shows poor sensitivity and specificity, whereas the ‘gold standard’ toxigenic culture is laborious and time consuming. Molecular tests for CDI are fast and highly sensitive and specific. Our laboratory has developed a molecular procedure that allows for r...
متن کاملIdentification of ryanodine receptor 1 single-nucleotide polymorphisms by high-resolution melting using the LightCycler 480 System.
High-resolution melting (HRM) allows single-nucleotide polymorphism (SNP) detection/typing using inexpensive generic heteroduplex-detecting double-stranded DNA (dsDNA) binding dyes. Until recently HRM has been a post-PCR process. With the LightCycler 480 System, however, the entire mutation screening process, including post-PCR analysis, can be performed using a single instrument. HRM assays we...
متن کاملHigh-throughput SNP Genotyping by Melting Curve Analysis for Resistance to Southern Root-knot Nematode and Frogeye Leaf Spot in Soybean
Melting curve analysis of fluorescently labeled DNA fragments is used extensively for genotyping single nucleotide polymorphism (SNP). Here, we evaluated a SNP genotyping method by melting curve analysis with the two probe chemistries in a 384-well plate format on a Roche LightCycler 480. The HybProbe chemistry is based on the fluorescence resonance energy transfer (FRET) and the SimpleProbe ch...
متن کامل3250 LC 480 TN 1.indd
High resolution melting (HRM) is a novel, closed-tube, post-PCR technique allowing genomic researchers to easily analyze genetic variations in PCR amplicons. This technical note describes general steps of setting up HRM-based PCR assays, with a special focus on ways how to optimize procedures for gene scanning experiments. The novel High Resolution Melting (HRM) technique enables researchers to...
متن کامل